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Spit Camp I Agenda

DAY 1: Lecture and Pre-Analytical Lab Practicum

8:30 am – 2:30 pm: Lecture with Doug Granger

  • Welcome and Introductions
    • Overview of Agenda and Activities
    • Theoretical Assumptions and Models
  • Oral Fluids as Biological Specimens
    • Analytes of Interest in Oral Fluid
  • Saliva Collection, Handling, and Storage
  • Research Design and Sampling Schemes

12:00 pm – 12:45 pm: Lunch Break

12:45 pm – 2:15 pm: Lecture with Doug Granger

  • Basics of Salivary Assays
  • Salivary Analyte Data Issues

Break

2:30 pm – 4:30 pm: Pre-Analytical Lab Practicum with IISBR Lab Team

 Introduction to Laboratory equipment and Safety Training

  • Introduction to the fundamentals of pipetting
  • Volume Recovery Demonstration

Break

  • Specimen Collection
  • Pipetting Practice Session
  • Sample Prep and Overnight Storage

Break

Before Bed: Assay Review Homework and First Sample Collection (SOS/Bedtime)

DAY 2: Analytical Lab Practicum and Data Discussion

Upon Waking: Second Sample collection (SOS/wake)

30 Min After Waking: Third Sample collection (SOS/+ 30)

8:30 am: Sample Drop Off

9:00 am – 12:00 am: Analytical Lab with IISBR Lab Team 

  • Sample Preparation
  • Cortisol Assay Training part 1

Break

  • Cortisol Assay Training part 2

12:00 pm – 12:45 pm: Lunch Break and Discussion with Doug Granger and Jenna Riis 

  • Lunch
  • Open Discussion

12:45 pm – 2:45 pm: Analytical Lab with IISBR Lab Team

  • Alpha Amylase Assay Training

Break

2:45 pm – 5:00 pm: Discussion with Doug Granger and IISBR Lab Team

  • Cortisol Data Discussion
  • Alpha Amylase Data Discussion
  • Conclusion
  • Q&A

5:00 pm – 5:15 pm: End of the Camp with Doug Granger and IISBR Lab Team

**PLEASE NOTE: LAB ATTIRE

While in the laboratory, you will be provided with laboratory coats to wear.  No shorts or open-toed shoes are allowed for safety purposes.  Please plan your attire accordingly.

DAY 3 (optional): Pre-analytic Data Processing and Basic Statistical Approaches

8:30 am – 10:00 pm: Understanding and importing data reported from the lab - Lecture and guided practice with Jenna Riis  

  • Review of lab-related data measures (e.g., calculating inter- and intra-assay CV, flow rate)
  • Review of lab notes (e.g., contamination; dilution)
  • Importing data into a statistical program

Break

10:15 am – 12:00 pm: Examining data quality and characteristics- Lecture with Jenna Riis

12:00 - 12:45 pm: Lunch Break and Discussion with Jenna Riis

12:45 pm – 2:15 pm: Examining data quality and characteristics- Guided Practice with Jenna Riis  

  • Missingness- causes and approaches
  • Data distributions
  • Identifying and addressing outliers

Break

2:30 pm – 5:00 pm: Computing and analyzing composite measures- Lecture and Guided Practice with Jenna Riis

Contact Us with any additional questions.

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Recent Publications

  • Kimonis, E. R., et al. (2018). Dehydroepiandrosterone (DHEA) and its ratio to cortisol moderate associations between maltreatment and psychopathology in male juvenile offenders. Psychoneuroendocrinology.
  • Gatzke-Kopp, L. M., et al. (2018). Magnitude and Chronicity of Environmental Smoke Exposure Across Infancy and Early Childhood in a Sample of Low-Income Children. Nicotine Tob Res.
  • Pisanic, N., et al. (2018). Minimally Invasive Saliva Testing to Monitor Norovirus Infection in Community Settings. J Infect Dis.
  • Affifi, T. D., et al. (2018). Testing the theory of resilience and relational load (TRRL) in families with type I diabetes. Health Commun.
  • Wheelock, M.D., et al. (2018). Psychosocial stress reactivity is associated with decreased whole brain network efficiency and increased amygdala centrality. Behav Neurosci.
  • Kornienko, O., et al. (2018). Associations Between Secretory Immunoglobulin A and Social Network Structure. Int J Behav Med.
  • Kuhlman, K. R., et al. (2018). Interparental conflict and child HPA-axis responses to acute stress: Insights using intensive repeated measures. J Fam Psychol.
  • Kuhlman, K. R., et al. (2018). HPA-Axis Activation as a Key Moderator of Childhood Trauma Exposure and Adolescent Mental Health. Journal of abnormal child psychology.
  • Corey-Bloom, J., et al. (2018). Salivary levels of total huntingtin are elevated in Huntington’s disease patients. Sci Rep.
  • Martinez, A. D., et al. (2018). Household fear of deportation in Mexican-origin families: Relation to body mass index percentiles and salivary uric acid. Am J Hum Biol.
  • Lucas, T., et al. (2018). Justice for all? Beliefs about justice for self and others and telomere length in African Americans. Cultur Divers Ethnic Minor Psychol.
  • Woerner, J., et al. (2018). Salivary uric acid: Associations with resting and reactive blood pressure response to social evaluative stress in healthy African Americans. Psychoneuroendocrinology.
  • Riis J.L., et al. (2018). The validity, stability, and utility of measuring uric acid in saliva. Biomark Med.

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Social Ecology I
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www.uci.edu
www.socialecology.uci.edu

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