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Spit Camp I Agenda

DAY 1: Lecture and Pre-Analytical Lab Practicum

8:30 am – 2:30 pm: Lecture with Drs. Beth Thomas and Michael Hoyt

  • Welcome and Introductions
    • Overview of Agenda and Activities
    • Theoretical Assumptions and Models
  • Oral Fluids as Biological Specimens
    • Analytes of Interest in Oral Fluid
  • Saliva Collection, Handling, and Storage
  • Research Design and Sampling Schemes

12:00 pm – 12:45 pm: Lunch Break

12:45 pm – 2:15 pm: Lecture with Drs. Beth Thomas and Michael Hoyt

  • Basics of Salivary Assays
  • Salivary Analyte Data Issues

Break

2:30 pm – 4:30 pm: Pre-Analytical Lab Practicum with IISBR Lab Team

 Introduction to Laboratory equipment and Safety Training

  • Introduction to the fundamentals of pipetting
  • Volume Recovery Demonstration

Break

  • Specimen Collection
  • Pipetting Practice Session
  • Sample Prep and Overnight Storage

Break

Before Bed: Assay Review Homework and First Sample Collection (SOS/Bedtime)

DAY 2: Analytical Lab Practicum and Data Discussion

Upon Waking: Second Sample collection (SOS/wake)

30 Min After Waking: Third Sample collection (SOS/+ 30)

8:30 am: Sample Drop Off

9:00 am – 12:00 am: Analytical Lab with IISBR Lab Team 

  • Sample Preparation
  • Cortisol Assay Training part 1

Break

  • Cortisol Assay Training part 2

12:00 pm – 12:45 pm: Lunch Break and Discussion with Drs. Beth Thomas and Michael Hoyt

  • Lunch
  • Open Discussion

12:45 pm – 2:45 pm: Analytical Lab with IISBR Lab Team

  • Alpha Amylase Assay Training

Break

2:45 pm – 5:00 pm: Discussion with Drs. Beth Thomas and Michael Hoyt and IISBR Lab Team

  • Cortisol Data Discussion
  • Alpha Amylase Data Discussion
  • Conclusion
  • Q&A

5:00 pm – 5:15 pm: End of the Camp with Drs. Beth Thomas and Michael Hoyt and IISBR Lab Team

**PLEASE NOTE: LAB ATTIRE

While in the laboratory, you will be provided with laboratory coats to wear.  No shorts or open-toed shoes are allowed for safety purposes.  Please plan your attire accordingly.

DAY 3 (optional): Pre-analytic Data Processing and Basic Statistical Approaches

8:30 am – 10:00 pm: Understanding and importing data reported from the lab – Lecture and guided practice  

  • Review of lab-related data measures (e.g., calculating inter- and intra-assay CV, flow rate)
  • Review of lab notes (e.g., contamination; dilution)
  • Importing data into a statistical program

Break

10:15 am – 12:00 pm: Examining data quality and characteristics- Lecture

12:00 – 12:45 pm: Lunch Break and Discussion

12:45 pm – 2:15 pm: Examining data quality and characteristics- Guided Practice

  • Missingness- causes and approaches
  • Data distributions
  • Identifying and addressing outliers

Break

2:30 pm – 5:00 pm: Computing and analyzing composite measures- Lecture and Guided Practice

Contact Us with any additional questions.

Pre-Register

UCI School of Social Ecology
Social Ecology I
Irvine, CA 92697-7050
www.uci.edu
www.socialecology.uci.edu

UCI Program in Public Health
UCI Health Sciences Complex
856 Health Sciences Quad
Irvine, CA 92697-3957
www.uci.edu www.publichealth.uci.edu

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